Journal: Frontiers in Microbiology

Article Title: Selective Isolation of Multidrug-Resistant Pedobacter spp., Producers of Novel Antibacterial Peptides

doi: 10.3389/fmicb.2021.642829

Figure Lengend Snippet: Isolated Pedobacter strains, antibiotics used for their selection, closest established identity according to 16S rRNA gene sequences (GenBank accession numbers MW332355 to MW332382 ), and sample collection coordinates.

Article Snippet: Sequencing was also performed for the five type isolates of different Pedobacter species purchased from the Belgian Co-ordinated Collections of Micro-organisms [BCCM/LMG, Pedobacter cryoconitis A37 (LMG 21415 T ) and P. lusitanus NL19 (LMG 29220 T )], the German Collection of Microorganisms and Cell Cultures [DSMZ, Pedobacter hartonius WB 3.3-3 (DSM 19033 T ) and Pedobacter westerhofensis WB 3.3-22 (DSM 19036 T )] as well as from the Japan Collection of Microorganisms [JCM, Pedobacter himalayensis HHS22 (JCM 12171 T )].

Techniques: Isolation, Selection

Journal: Frontiers in Microbiology

Article Title: Selective Isolation of Multidrug-Resistant Pedobacter spp., Producers of Novel Antibacterial Peptides

doi: 10.3389/fmicb.2021.642829

Figure Lengend Snippet: A graphical representation of the relative abundance of the one hundred most common microbial OTUs (operational taxonomic units) at genus level in sample 10 . These OTUs correspond to 76% of the total number of reads. The indicated slice representing the genus Pedobacter represents 0.32% or 93 individual reads out of 29,122.

Article Snippet: Sequencing was also performed for the five type isolates of different Pedobacter species purchased from the Belgian Co-ordinated Collections of Micro-organisms [BCCM/LMG, Pedobacter cryoconitis A37 (LMG 21415 T ) and P. lusitanus NL19 (LMG 29220 T )], the German Collection of Microorganisms and Cell Cultures [DSMZ, Pedobacter hartonius WB 3.3-3 (DSM 19033 T ) and Pedobacter westerhofensis WB 3.3-22 (DSM 19036 T )] as well as from the Japan Collection of Microorganisms [JCM, Pedobacter himalayensis HHS22 (JCM 12171 T )].

Techniques:

Journal: Frontiers in Microbiology

Article Title: Selective Isolation of Multidrug-Resistant Pedobacter spp., Producers of Novel Antibacterial Peptides

doi: 10.3389/fmicb.2021.642829

Figure Lengend Snippet: NJ tree (Jukes–Cantor/bootstrap 1000) based on 16S rRNA gene sequence analysis showing the phylogenetic relationship between the isolated Pedobacter strains and five closely related species of Pedobacter that are available from culture collections (GenBank accession numbers MW332350 to MW332382 ). The online multiple alignment program for amino acid or nucleotide sequences, MAFFT version 7, was used for the alignment and subsequent tree building .

Article Snippet: Sequencing was also performed for the five type isolates of different Pedobacter species purchased from the Belgian Co-ordinated Collections of Micro-organisms [BCCM/LMG, Pedobacter cryoconitis A37 (LMG 21415 T ) and P. lusitanus NL19 (LMG 29220 T )], the German Collection of Microorganisms and Cell Cultures [DSMZ, Pedobacter hartonius WB 3.3-3 (DSM 19033 T ) and Pedobacter westerhofensis WB 3.3-22 (DSM 19036 T )] as well as from the Japan Collection of Microorganisms [JCM, Pedobacter himalayensis HHS22 (JCM 12171 T )].

Techniques: Sequencing, Isolation

Journal: Frontiers in Microbiology

Article Title: Selective Isolation of Multidrug-Resistant Pedobacter spp., Producers of Novel Antibacterial Peptides

doi: 10.3389/fmicb.2021.642829

Figure Lengend Snippet: Top: Heat map generated by hierarchical clustering (Euclidean, complete) of major compounds (rows) produced by 33 strains of Pedobacter (columns) as analyzed by UHPLC-MS. Numbers indicate clustering of compounds discussed in the text. [UP-strains are from this work. P. cry. , Pedobacter cryoconitis A37 (LMG 21415 T ); P. lus. , P. lusitanus NL19 (LMG 29220 T ); P. har. , P. hartonius WB 3.3-3 (DSM 19033 T ); P. wes. , P. westerhofensis WB 3.3-22 (DSM 19036 T ); P. him ., P. himalayensis HHS22 (JCM 12171 T ).] Bottom: Chromatograms (A210) from HPLC-fractionation of the indicated Pedobacter isolates, with data from bioassays against a panel of bacterial pathogens under each chromatogram. Bacteria are shown in rows (from top to bottom: E. coli, A. baumannii , E. cloacae , K. pneumoniae , P. aeruginosa , and S. aureus ) and HPLC fractions in columns. Activity grading of fractions is ranging from red (full inhibition) to white (no visible inhibition). The presence of peptides from clusters 1–14 is indicated in each chromatogram.

Article Snippet: Sequencing was also performed for the five type isolates of different Pedobacter species purchased from the Belgian Co-ordinated Collections of Micro-organisms [BCCM/LMG, Pedobacter cryoconitis A37 (LMG 21415 T ) and P. lusitanus NL19 (LMG 29220 T )], the German Collection of Microorganisms and Cell Cultures [DSMZ, Pedobacter hartonius WB 3.3-3 (DSM 19033 T ) and Pedobacter westerhofensis WB 3.3-22 (DSM 19036 T )] as well as from the Japan Collection of Microorganisms [JCM, Pedobacter himalayensis HHS22 (JCM 12171 T )].

Techniques: Generated, Produced, Fractionation, Bacteria, Activity Assay, Inhibition

Journal: International journal of systematic and evolutionary microbiology

Article Title: Pedobacter duraquae sp. nov., Pedobacter westerhofensis sp. nov., Pedobacter metabolipauper sp. nov., Pedobacter hartonius sp. nov. and Pedobacter steynii sp. nov., isolated from a hard-water rivulet.

doi: 10.1099/ijs.0.65166-0

Figure Lengend Snippet: Fig. 1. Relationship of the WB isolates to type strains of Pedobacter species. The dendro- gram is based on 16S rRNA gene sequence comparisons using the algorithm of De Soete (1983). Bar, 2 % sequence divergence, as determined by measuring the length of the horizontal lines connecting any two species.

Article Snippet: Pedobacter duraquae sp. nov., Pedobacter westerhofensis sp. nov., Pedobacter metabolipauper sp. nov., Pedobacter hartonius sp. nov. and Pedobacter steynii sp. nov., isolated from a hard-water rivulet Sören Muurholm, Sylvie Cousin, Orsola Päuker, Evelyne Brambilla and Erko Stackebrandt Correspondence Sylvie Cousin sylvie.cousin@dsmz.de DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstrasse 7b, D-38124 Braunschweig, Germany Five isolates that were related phylogenetically to members of the genus Pedobacter were isolated from freshwater of the hard-water creek Westerhöfer Bach, North Germany.

Techniques: Sequencing

Journal: International journal of systematic and evolutionary microbiology

Article Title: Pedobacter duraquae sp. nov., Pedobacter westerhofensis sp. nov., Pedobacter metabolipauper sp. nov., Pedobacter hartonius sp. nov. and Pedobacter steynii sp. nov., isolated from a hard-water rivulet.

doi: 10.1099/ijs.0.65166-0

Figure Lengend Snippet: Fig. 2. Dendrogram showing the fatty acid relationships among the WB isolates and phylogenetically neighbouring Pedobacter reference strains. The dendrogram was gener- ated by treating the Euclidian distances of the fatty acids with the unweighted pair group method with arithmetic means algorithm. Numerical analyses were done using standard MIS software (Microbial ID).

Article Snippet: Pedobacter duraquae sp. nov., Pedobacter westerhofensis sp. nov., Pedobacter metabolipauper sp. nov., Pedobacter hartonius sp. nov. and Pedobacter steynii sp. nov., isolated from a hard-water rivulet Sören Muurholm, Sylvie Cousin, Orsola Päuker, Evelyne Brambilla and Erko Stackebrandt Correspondence Sylvie Cousin sylvie.cousin@dsmz.de DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstrasse 7b, D-38124 Braunschweig, Germany Five isolates that were related phylogenetically to members of the genus Pedobacter were isolated from freshwater of the hard-water creek Westerhöfer Bach, North Germany.

Techniques: Software

Journal: International journal of systematic and evolutionary microbiology

Article Title: Micromonospora lupini sp. nov. and Micromonospora saelicesensis sp. nov., isolated from root nodules of Lupinus angustifolius.

doi: 10.1099/ijs.0.65192-0

Figure Lengend Snippet: Fig. 1. Neighbour-joining tree based on 16S rRNA gene sequences showing the relation- ships among the six new isolates and the phylogenetically closest Micromonospora spe- cies. Bootstrap percentages (from 1000 repli- cates) above 50 % are shown at nodes. Bar, 0.005 substitutions per nucleotide position. An extended version of this tree containing all species of Micromonospora is available as Supplementary Fig. S2.

Article Snippet: Micromonospora lupini sp. nov. and Micromonospora saelicesensis sp. nov., isolated from root nodules of Lupinus angustifolius Martha E. Trujillo,1 Reiner M. Kroppenstedt,2 Carmen FernándezMolinero,1 Peter Schumann2 and Eustoquio Martı́nez-Molina1 Correspondence Martha E. Trujillo mett@usal.es 1Departamento de Microbiologı́a y Genética, Edificio Departamental, Lab. 209, Campus Miguel de Unamuno, Universidad de Salamanca, Salamanca, Spain 2DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, 38124 Braunschweig, Germany A study was conducted to determine the taxonomic status of six actinomycete strains isolated from root nodules of Lupinus angustifolius.

Techniques:

Journal: International journal of systematic and evolutionary microbiology

Article Title: Micromonospora lupini sp. nov. and Micromonospora saelicesensis sp. nov., isolated from root nodules of Lupinus angustifolius.

doi: 10.1099/ijs.0.65192-0

Figure Lengend Snippet: Fig. 2. Riboprint patterns of Micromonospora isolates generated with the restriction enzyme PvuII.

Article Snippet: Micromonospora lupini sp. nov. and Micromonospora saelicesensis sp. nov., isolated from root nodules of Lupinus angustifolius Martha E. Trujillo,1 Reiner M. Kroppenstedt,2 Carmen FernándezMolinero,1 Peter Schumann2 and Eustoquio Martı́nez-Molina1 Correspondence Martha E. Trujillo mett@usal.es 1Departamento de Microbiologı́a y Genética, Edificio Departamental, Lab. 209, Campus Miguel de Unamuno, Universidad de Salamanca, Salamanca, Spain 2DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, 38124 Braunschweig, Germany A study was conducted to determine the taxonomic status of six actinomycete strains isolated from root nodules of Lupinus angustifolius.

Techniques: Generated